Journal: Science Advances
Article Title: Networks of habenula-projecting cortical neurons regulate cocaine seeking
doi: 10.1126/sciadv.abj2225
Figure Lengend Snippet: ( A ) Summary of MAPseq procedure. ( B ) Percentage of barcodes detected in the mPFC also detected in brain sites listed on y axis. Data were collected from n = 2 mice. ( C ) Heatmap representation of mPFC barcodes (% total number) detected in brain regions shown on y axis and in regions shown on x axis. ( D ) Histogram of connectome of mPFC ➔PVT , mPFC ➔VTA , and mPFC ➔LHb neurons. Shown is the distribution of barcodes (%) for mPFC cells that project to the PVT, VTA, and LHb. ( E ) Summary of injection procedure to label cortical-habenular neurons that project to VTA and fluorescence image of mPFC from mice injected with CTb retrograde tracers in the LHb and VTA. Shown are CTb-594 (red; mPFC ➔LHb neurons)–labeled and CTb-488 (green; mPFC ➔VTA neurons)–labeled cells. White arrows identify dual-labeled cells (yellow; mPFC ➔LHb VTA neurons) in the mPFC. A total of n = 5 mice were imaged. ( F ) Summary of injection procedure to label cortical-habenular neurons that project to the LC and associated fluorescence image of mPFC from injected mice (red; mPFC ➔LHb neurons) and GFP (green; mPFC ➔VTA neurons). White arrows identify dual-labeled cells (yellow; mPFC ➔LHb LC neurons) in the mPFC. A total of n = 5 mice were imaged. ( G ) Injection procedure (the tracing the relationship between input and output method, also known as the TRIO method) to label cortico-habenular neurons that project to VTA. Shown are mCherry + cells in the mPFC, which are cortico-habenular neurons that synapse into LHb neurons that project to VTA. A total of n = 4 mice were imaged. ( H ) Injection procedure to label cortico-habenular neurons that project to the LC. mCherry + cells were detected in the mPFC (right), which are cortico-habenular neurons that synapse into LHb neurons that project to the LC. A total of n = 3 mice were imaged. ( I ) Graphical representation of the cortico-habenular connectome. A population of mPFC neurons (shown in blue) projects concurrently to the LHb and then to the same monoaminergic brain centers to which LHb neurons also project (shown in red).
Article Snippet: This was accomplished by injecting the MAPseq virus (Sindbis virus, Cold Spring Harbor Laboratories) into the mPFC.
Techniques: Injection, Fluorescence, Labeling
